Dr. Wei-Hua Wu
Dr. Wureceived her Ph.D. in genetics and molecular biology from the University of Medicine and Dentistry of New Jersey. She did postdoctoral training in chromatin remodeling at the National Cancer Institute. She is an assistant professor in the Institute of Molecular Medicine and Genetics and Department of Neurology in the Medical College of Georgia at GHSU.
Dr. Wu’s research focuses on understanding the biological functions of ATP-dependent chromatin remodeling.
Chromatin is a highly condensed and complex organization of eukaryotic DNA and histone proteins. The structure of chromatin is essential to control DNA metabolism and gene expression. ATP-dependent chromatin remodeling enzymes, a class of evolutionarily conserved enzymes, plays a key role in regulating the ‘on’ and ‘off’ states of chromatin. It changes chromatin structure to establish a dynamic molecular interface, thereby facilitating the accessibility of large DNA enzymes, histone modification enzymes or regulatory factors. Misregulation of this process may cause a cascade of pathologic events including disordered gene expression, failed DNA damage response, and genome instability, all of which may lead to cancer. Using a combination of the powerful modern yeast molecular biology, biochemistry and functional genomics techniques, the Wu laboratory studies how an ATP-dependent chromatin remodeling enzyme, Fun30/Smarcad1, alters chromatin structure to regulate transcription, repair and replication.
Wu WH*, Wu CH, Ladurner A, Mizuguchi G, Wei D, Xiao H, Luk L, Ranjan A and Wu C*. N-terminus of Swr1 binds to histone H2AZ and provides a platform for subunit assembly in the chromatin remodeling complex. J Biol Chem 284, 6200-6207 (2009). Published online December 16, 2008. *corresponding authors.
Luk E, Vu ND, Patteson K, Mizuguchi G, Wu WH, Ranjan A, Backus J, Sen S, Lewis M, Bai Y, Wu C. Chz1, a nuclear chaperone for histone H2AZ. Mol Cell 25, 357-368 (2007).
Mizuguchi G, Shen X, Landry J, Wu WH, Sen S, and Wu C. ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex. Science 303, 343-348 (2004). Published Online November 27, 2003.
Shen X., Xiao H, Ranallo RT, Wu WH, and Wu C. Inositol polyphosphates modulate ATP-dependent chromatin remodeling complexes. Science 299, 112-114 (2003). Published online November 14, 2002
Wu WH and Hampsey M. An activation-specific role for transcription factor TFIIB in vivo. Proc. Natl. Acad. Sci. 96, 2764-2769 (1999).
Wu WH, Pinto I, Chen BS, and Hampsey M. Mutational analysis of yeast TFIIB: a functional relationship between Ssu72 and Sub1/Tsp1 defined by allele-specific interactions with TFIIB. Genetics 153, 643-652(1999).
Orphanides G, Wu WH, Lane WS, Hampsey M, and Reinberg D. The chromatin-specific transcription elongation factor FACT comprises the SPT16/CDC68 and SSRP1 proteins. Nature 400, 284-288 (1999).
Wu WH and Hampsey M. Transcription: Common cofactors and cooperative recruitment . Current Biology 9, 606-609 (1999).
Pinto I, Wu WH, Na JG, and Hampsey M. Characterization of sua7 mutants defines a domain of TFIIB involved in transcription start site selection in yeast. J. Biol. Chem. 269, 30569-30573 (1994).